Comments on: Basics: Sequencing DNA, Part 2 http://www.genetic-inference.co.uk/blog/2009/08/basics-sequencing-dna-part-2/ Sun, 22 Jan 2012 22:30:33 +0000 hourly 1 http://wordpress.org/?v=3.2.1 By: Jimmy http://www.genetic-inference.co.uk/blog/2009/08/basics-sequencing-dna-part-2/comment-page-1/#comment-42409 Jimmy Fri, 28 Oct 2011 14:57:01 +0000 http://www.genetic-inference.co.uk/blog/?p=413#comment-42409 Thanks a lot for this. Helped me so much understand something that may official textbooks and lectures could not. It's clear and understandable. Thank you very much Thanks a lot for this. Helped me so much understand something that may official textbooks and lectures could not. It’s clear and understandable. Thank you very much

]]> By: Luke http://www.genetic-inference.co.uk/blog/2009/08/basics-sequencing-dna-part-2/comment-page-1/#comment-4156 Luke Fri, 21 May 2010 23:29:46 +0000 http://www.genetic-inference.co.uk/blog/?p=413#comment-4156 Yeah of course that should have read $40-50k. The price (for a sequencing facility) is down to around $10k for a high quality genome sequence now, and will probably fall to closer to $5k by the end of the year. I never wrote a "Basics" 3rd Gen post, though I did write a more technical low-down on 3rd gen technology a while ago: http://www.genetic-inference.co.uk/blog/?p=839 I'd love to write more sequencing basics posts, but I'm currently working on a different project, so they may be a while in coming. Yeah of course that should have read $40-50k. The price (for a sequencing facility) is down to around $10k for a high quality genome sequence now, and will probably fall to closer to $5k by the end of the year.

I never wrote a “Basics” 3rd Gen post, though I did write a more technical low-down on 3rd gen technology a while ago:

http://www.genetic-inference.co.uk/blog/?p=839

I’d love to write more sequencing basics posts, but I’m currently working on a different project, so they may be a while in coming.

]]> By: Callan http://www.genetic-inference.co.uk/blog/2009/08/basics-sequencing-dna-part-2/comment-page-1/#comment-3910 Callan Thu, 13 May 2010 15:21:00 +0000 http://www.genetic-inference.co.uk/blog/?p=413#comment-3910 Nice explaination. could you just clarify the "However, second gen sequencing is not without its flaws. While it has got cheap ($40-50 to sequence a human genome these days)" Then you say "Third Generation Sequencing machines, which promise to make sequencing an entire human genome cost a couple of hundred pounds and take a few hours." I realise $40-50 is far to low, but how much does it cost to sequence a (whole) human genome at the moment approximately? Oh and did you write 'part 3' yet? Thanks Nice explaination. could you just clarify the
“However, second gen sequencing is not without its flaws. While it has got cheap ($40-50 to sequence a human genome these days)”
Then you say
“Third Generation Sequencing machines, which promise to make sequencing an entire human genome cost a couple of hundred pounds and take a few hours.”
I realise $40-50 is far to low, but how much does it cost to sequence a (whole) human genome at the moment approximately?
Oh and did you write ‘part 3′ yet?
Thanks

]]> By: Neni http://www.genetic-inference.co.uk/blog/2009/08/basics-sequencing-dna-part-2/comment-page-1/#comment-1120 Neni Wed, 11 Nov 2009 10:04:29 +0000 http://www.genetic-inference.co.uk/blog/?p=413#comment-1120 looking forward to your 3rd part! looking forward to your 3rd part!

]]> By: kurye http://www.genetic-inference.co.uk/blog/2009/08/basics-sequencing-dna-part-2/comment-page-1/#comment-550 kurye Mon, 24 Aug 2009 18:02:00 +0000 http://www.genetic-inference.co.uk/blog/?p=413#comment-550 Basics: Sequencing DNA, Part 2 « Genetic Inference great article thank you. Basics: Sequencing DNA, Part 2 « Genetic Inference great article thank you.

]]> By: Luke http://www.genetic-inference.co.uk/blog/2009/08/basics-sequencing-dna-part-2/comment-page-1/#comment-516 Luke Tue, 18 Aug 2009 08:16:43 +0000 http://www.genetic-inference.co.uk/blog/?p=413#comment-516 There you go; I've added a brief footnote with some error rate references. There you go; I’ve added a brief footnote with some error rate references.

]]> By: Thomas Keller http://www.genetic-inference.co.uk/blog/2009/08/basics-sequencing-dna-part-2/comment-page-1/#comment-507 Thomas Keller Mon, 17 Aug 2009 18:01:28 +0000 http://www.genetic-inference.co.uk/blog/?p=413#comment-507 Nice exposition, and amusing "cabal thing" notes. But I was wondering if you could include references, or methodology, for the stated error rates. This is an important issue for me, as a core facility director talking to clients about the technology. -- thanks. Nice exposition, and amusing “cabal thing” notes. But I was wondering if you could include references, or methodology, for the stated error rates. This is an important issue for me, as a core facility director talking to clients about the technology. — thanks.

]]> By: Twitted by fschlegel http://www.genetic-inference.co.uk/blog/2009/08/basics-sequencing-dna-part-2/comment-page-1/#comment-486 Twitted by fschlegel Fri, 14 Aug 2009 14:45:29 +0000 http://www.genetic-inference.co.uk/blog/?p=413#comment-486 [...] This post was Twitted by fschlegel [...] [...] This post was Twitted by fschlegel [...]

]]> By: John http://www.genetic-inference.co.uk/blog/2009/08/basics-sequencing-dna-part-2/comment-page-1/#comment-483 John Thu, 13 Aug 2009 21:15:13 +0000 http://www.genetic-inference.co.uk/blog/?p=413#comment-483 Incredible explanation........thanks. Incredible explanation……..thanks.

]]>